Bioinoculants influence accumulation of phytochemicals in Oroxylum indicum (L.) Benth. ex Kurz seedlings

Abstract

The seedlings of Oroxylum indicum were inoculated with plant growth promoting microbes (PGPMs) mainly, Pseudomonas putida, Trichoderma harzianum and Glomus mosseae both alone and consortium. The preliminary qualitative and quantitative phytochemical analysis of the root extract of the inoculated seedlings of O. indicum showed the presence of carbohydrates, protein, saponins, tannins, alkaloids, phenols, flavonoids, terpenoids, and glycosides. The tannin concentration was found maximum (0.0500±0.037 mg/g) in TM (mycorrhizal treatment), while it was minimum (0.0341±0.321 mg/g) in non-inoculated/control treatment. Maximum (0.993±0.006 mg/g) chlorophyll content was present in TMB (Mycorrhizal + Bacterial treatment) and minimum (0.639±0.01 mg/g) in control treatment. The total alkaloid content was maximum (0.769±0.031) in TMB (Mycorrhizal + Bacterial treatment) while it was minimum (0.115±0.055) in control treatment. The total protein content was maximum (960.65±0.065 mg/g) in TMBF (Mycorrhizal + Bacterial + Fungal treatment) while it was minimum (299.04±0.0943 mg/g) in control treatment. The total phenolic content (TPC) was maximum (29.271± 0.388 mg/g in GAE) in TB (Bacterial treatment) while TPC was minimum (3.1±1.01 mg/g in GAE) in Control treatment. It can be concluded that the bioinoculants whether alone or in consortial form influences the phytochemicals in O. indicum but the consortial form has significant influence than alone treatments.

Keywords: Pseudomonas putida, Trichoderma harzianum and Glomus mosseae, bioinoculants, phytochemicals

Published by Dr. Chandrima Debi

Hi, I am Chandrima Debi. I am a Doctorate in Forestry and an independent researcher. Ever since childhood, I experienced deep-rooted connection with nature, forest and wildlife. I have written various research articles, case studies based on geology, forests, medicinal plants, biodiversity and conservation. Through this blog I share my experiences with nature and forests around us and aid towards the protection and conservation of biodiversity, wildlife and the values associated.

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